ISH control

ELADER at AMBION.COM ELADER at AMBION.COM
Fri Jun 19 08:40:48 EST 1998


In article <Eus28w.2B4 at riker.neoucom.edu>,
  rjv at riker.neoucom.edu (Richard Vernell) wrote:
>
>
> My question is:
>
> What is the best control for in situ hybridization with RNA probes.  Is an
> irrelavent probe as valid as the sense probe?

An unrelated sequence does not control for gc rich regions or repetitive
sequences found in a specific probe. Go with the sense strand probe.

Eric

-----== Posted via Deja News, The Leader in Internet Discussion ==-----
http://www.dejanews.com/   Now offering spam-free web-based newsreading



More information about the Methods mailing list