ISH control

[ELADER at AMBION.COM]

In article <Eus28w.2B4 at riker.neoucom.edu>,
  rjv at riker.neoucom.edu (Richard Vernell) wrote:
>
>
> My question is:
>
> What is the best control for in situ hybridization with RNA probes.  Is an
> irrelavent probe as valid as the sense probe?

An unrelated sequence does not control for gc rich regions or repetitive
sequences found in a specific probe. Go with the sense strand probe.

Eric

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