unfavorable human sequence to rodent(mouse/hamster/rat)

Jun-Ichi Aikawa jaikawa at UCSD.EDU
Fri Jun 19 07:15:57 EST 1998


Does anyone know information about unfavorable human sequence to
rodent(mouse/hamster/rat)? Is there human sequence which is hard to
transcribe or translate in rodent cell lines (e.g. CHO, NIH3T3)? In my
case, expression of human cDNA which contains short 5'- and 3'-untranslated
region seemed to be weak, although mouse cDNA of its family gene expressed
efficiently. GIve me any suggestions. Thanks.

Jun-ichi Aikawa, Ph.D.
University of California, San Diego





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