Stable Amino Acid Derivative for HPLC?

rpescev at rpescev at
Fri Jun 19 13:36:22 EST 1998

In article <3576E5D3.2290 at>,
  aweert at wrote:
> Please check out this URL: or email
rp at
Thanks for your time
> Phil Calvert wrote:
> >
> > There a number of methods available for derivatizing amino acids and
> > analyzing them by reverse-phase HPLC on a C18 column.  Unfortunately, many
> > of these derivatives are not very stable.  Obviously, they are stable
> > enough for analytical purposes.  But I am interested in doing preparative
> > HPLC and isolating the purified amino acid derivatives; so if the
> > derivatives aren't very stable, that sort of defeats the purpose.
> >
> > I would like to use a UV detector for detecting the derivatized amino
> > acids.  Therefore, derivatives that are UV-absorbing (including fluorescent
> > derivatives) are preferred (provided that they are stable).  Anyone know of
> > any derivatives that fit these qualifications?
> >
> > Phil Calvert
> AFAI remember, derivatizing them with ortho-phtalaldehyde (the OPA
> reagent, or fluoraldehyde) is reasonably stable
> Marco

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