FISH control

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Fri Jun 19 17:46:11 EST 1998


Actually, a reversed (same base composition, but reversed order would
be great, wouldn't it? 

Wolfgang


> In article <Eus28w.2B4 at riker.neoucom.edu>,
>   rjv at riker.neoucom.edu (Richard Vernell) wrote:
> >
> >
> > My question is:
> >
> > What is the best control for in situ hybridization with RNA
> > probes.  Is an irrelavent probe as valid as the sense probe?
> 
> An unrelated sequence does not control for gc rich regions or
> repetitive sequences found in a specific probe. Go with the sense
> strand probe.
> 
> Eric
> 
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touch! -----                              Wolfgang Schechinger       
University of Tuebingen, Germany email: wgschech at med.uni-tuebingen.de
* wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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-----
usual disclaimers apply * This message is RNAse free - please don't touch!
-----                              
Wolfgang Schechinger         
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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