FISH control
Wolfgang Schechinger
wgschech at med.uni-tuebingen.de
Fri Jun 19 17:46:11 EST 1998
Actually, a reversed (same base composition, but reversed order would
be great, wouldn't it?
Wolfgang
> In article <Eus28w.2B4 at riker.neoucom.edu>,
> rjv at riker.neoucom.edu (Richard Vernell) wrote:
> >
> >
> > My question is:
> >
> > What is the best control for in situ hybridization with RNA
> > probes. Is an irrelavent probe as valid as the sense probe?
>
> An unrelated sequence does not control for gc rich regions or
> repetitive sequences found in a specific probe. Go with the sense
> strand probe.
>
> Eric
>
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usual disclaimers apply * This message is RNAse free - please don't
touch! ----- Wolfgang Schechinger
University of Tuebingen, Germany email: wgschech at med.uni-tuebingen.de
* wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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usual disclaimers apply * This message is RNAse free - please don't touch!
-----
Wolfgang Schechinger
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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