High GC PCR

jeremy m vanderweele jmvander at midway.uchicago.edu
Fri Jun 19 16:15:43 EST 1998


I'm having a lot of difficulty amplifying a high GC (82-90%) region using
the GC-melt kit from Clontech.  The region is also pretty repetitive,
which makes it difficult to get good primers.  Does anyone have a better
protocol to PCR high GC regions?  (Cheaper would also be nice--Clontech's
enzyme costs a fortune.)

Thanks,

Jeremy Veenstra-VanderWeele
University of Chicago



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