Q:DNase1 digestion before RT-PCR

Jun Zhang junz at sfu.ca
Fri Jun 19 12:24:44 EST 1998

Hi, Andy:

I use Ambion DNase1. After in vitro transcription, I use DNase1 to digest 
DNA template. The RNA samples were confirmed free of DNA contamination
with PCR. You can check their website: www.ambion.com

Good luck.

> An article in BioTechniques (22:1128-1132) last year talked about using
> Manganese in RT-PCR to eliminate artefacts resulting from DNase1 digestion.
> Unfortunately, the article didn't describe the other components of the
> buffer.  I have a number of RNA samples which I need to digest with DNase
> before RT-PCR. Does anyone have a DNase digestion protocol that they have
> tried (and works!) that they could let me have please?
> Many thanks,
> --
> Dr. Andy Bettany
> Research Scientist
> Institute of Grassland and Environmental Research
> Plas Gogerddan
> Wales, UK

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