TA cloning

Mounir Izallalen mounir at rsvs.ulaval.ca
Sun Jun 21 17:02:09 EST 1998


Hi,
You can use PCR products to TA clone them even if they were kept at -20.
By the way no need to buy the T-clone, you can make them. Digest you
vector (pGEM or pBSK) with EcoRV, take an aliquot and add to a normal PCR
reaction with only dT, incubate 72 for 2 hours, chloroform extract,
precipitate and Voila!

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Mounir Izallalen			
Recherche en Sciences de la Vie et de la Sante
Pavillon C.E. Marchand, Laboratoire 1133
Universite laval, Ste-Foy, PQ G1K 7P4 Canada
Tel (418) 656-2131 ext#6785 Fax (418) 656-7176

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