DNA quantitation

Arnoud van Vliet arnoud at vanvliet.u-net.com
Mon Jun 22 19:24:19 EST 1998


Just buy lambda DNA from any supplier (Pharmacia, Gibco), cut a known amount
with PstI or HindIII and load a known amount on gel. Easy to calculate the
amount in each band of the marker. Of course yo could buy the 1 kb ladder or
something similar if you got money to waste.

Arnoud

Liz Parks wrote in message <358E8446.B5590D29 at ncsu.edu>...
>I want to estimate the concentration of PCR products on an agarose gel,
>but I can't find anything to use as a standard.  I need linear, dsDNA
>that I can dilute or comes in different dilutions.  I have used
>something like this in the past, but I can't remember who the supplier
>was.  Can anyone help?
>





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