RT-PCR

Elena Ayaso e.ayaso at ucg.ie
Mon Jun 22 08:13:29 EST 1998


I am using oligo dT to do reverse transcription on RNA from embryos.
Then I use specific primers on the 1st strand cDNA I get no PCR product.
When I use specific primers to do the reverse trnscription it works. 
I think the oligo dT might not be working. I would like to know what
anneling tempeture I should use if I want to use 18mer oligo dT as a
primer to check that it's O.K.
Elena Ayaso
Thanks



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