TA cloning

R.A. Hastings RAH22 at le.ac.uk
Mon Jun 22 13:59:05 EST 1998


Hello,

It probably goes without saying, but you can also clone Pfu-amplified
products into T vectors (homemade or otherwise) by cleaning up the
initial Pfu PCR reaction, then tailing the product with Taq polymerase
and dATP.

Richard

-- 
Dr.R.A.Hastings
Cardiovascular Research Institute
Leicester Royal Infirmary
U.K.



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