RIPPA kit for RPA
qiacom at aol.com
Tue Jun 23 23:07:31 EST 1998
We now also use biotin-labeled riboprobes for RPA with good results.
Typically, less than 1% of the probe goes a long way. But the most common
problem seems to be bad probes - ie incomplete transcripts, degraded
transcripts, etc. As long as you gel purify (6% acrylamide/8M urea) yourprobe
first you should get clean RPA gels. Omega Biotek has a nifty kit for gel
purification of RNA probes.
More information about the Methods