X-Gal staining of transfected cells: background

P.C. peter.cherepanov at uz.kuleuven.ac.be
Tue Jun 23 06:50:54 EST 1998


Hi!

Sometimes when I do X-Gal staining of HeLa cells transfected 
with pCMVbeta I obtain blue cells in the mock sample. (this 
staining is not as strong as in the positive cells, but it 
makes a problem when transfection efficiencies are low)

Does any one know how to reduce this background staining ?
Thanks for any suggestions,

Peter.



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