X-Gal staining of transfected cells: background
P.C.
peter.cherepanov at uz.kuleuven.ac.be
Tue Jun 23 06:50:54 EST 1998
Hi!
Sometimes when I do X-Gal staining of HeLa cells transfected
with pCMVbeta I obtain blue cells in the mock sample. (this
staining is not as strong as in the positive cells, but it
makes a problem when transfection efficiencies are low)
Does any one know how to reduce this background staining ?
Thanks for any suggestions,
Peter.
More information about the Methods
mailing list