Urgent: can't resupend my DNA!!!!

Frank O. Fackelmayer fof1 at chclu.chemie.uni-konstanz.de
Tue Jun 23 05:20:17 EST 1998


Be patient. Redissolving genomic DNA must not be urgent. Let it stand at high
temperature overnight (65C) or longer, in TE. Final concentration should not
exceed 2mg/ml. Add more buffer if necessary. If you overdried your pellet, it
will be even worse to dissolve again. 

Hope this helps,
Frank

Urbani, Nicola wrote:
> 
> Does anybody know what to do when genomic DNA doesn't resupend after an
> ethanol precipitation in the presence of Sodium acetate?



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