resuspending high molecular weight genomic DNA: a problem?

drdad at ktc.com drdad at ktc.com
Wed Jun 24 05:33:17 EST 1998


While genomic DNA which is very pure might disappear from view in seconds,
it definitely takes hours to over night at 37-65oC to uniformly dissolve
into solution.  The purity of the DNA and the pH of the solution would
also affect the process.

Dr. Felix D. Guerrero
drdad at ktc.com

In article <35911BB8.4BA6D5F4 at chclu.chemie.uni-konstanz.de>,
fof1 at chclu.chemie.uni-konstanz.de wrote:

> Parent, Eric wrote:
> > 
> > Hello all.
> > 
> > I ha a simple question. I got in an argument (kind of) with a visiting
> > scientist in our lab.  He was telling me that is genomic DNA use to disolve
> > in seconds. My experience is that genomic always take some time to
> > dissolve(up to some days). Anybody had any experience with fast respension
> > of genomic DNA. Does the quality of  the phenol or the chloroform can make
> > the difference?
> 
> I´ve never seen genomic DNA (neither prokaryotic nor eukaryotic) dissolving in
> seconds. I agree with you that it takes hours (to overnight) at elevated
temperature.
> 
> Frank



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