Q: heteroduplex analysis

Wolfgang Resch wresch at email.unc.edu
Wed Jun 24 09:22:53 EST 1998


On Wed, 24 Jun 1998, Juergen Schmitz wrote:

> Hi netters!
> Currently I am trying to get heteroduplex analysis working.
> 
> My question is if anybody knows the composition of the native acrylamid
> gels used for heteroduplex analysis.
> I would like to avoid bying the expensive ready to go MDE acrylamid if
> possible.
> 
> Thanx
> 
> JuerGen

hi juergen,

we use acrylamide : bisacrylamide = 37.5 : 1 (that's basically protogel).
for my 250 bp fragment, i run 12.3 % T, 2.6 % C gels in TBE. works fine.

take care

wolf




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