resuspending high molecular weight genomic DNA: a problem?

[Frank O. Fackelmayer]

Parent, Eric wrote:
> 
> Hello all.
> 
> I ha a simple question. I got in an argument (kind of) with a visiting
> scientist in our lab.  He was telling me that is genomic DNA use to disolve
> in seconds. My experience is that genomic always take some time to
> dissolve(up to some days). Anybody had any experience with fast respension
> of genomic DNA. Does the quality of  the phenol or the chloroform can make
> the difference?

I´ve never seen genomic DNA (neither prokaryotic nor eukaryotic) dissolving in
seconds. I agree with you that it takes hours (to overnight) at elevated temperature.

Frank