resuspending high molecular weight genomic DNA: a problem?

Frank O. Fackelmayer fof1 at chclu.chemie.uni-konstanz.de
Wed Jun 24 10:30:54 EST 1998


Parent, Eric wrote:
> 
> Hello all.
> 
> I ha a simple question. I got in an argument (kind of) with a visiting
> scientist in our lab.  He was telling me that is genomic DNA use to disolve
> in seconds. My experience is that genomic always take some time to
> dissolve(up to some days). Anybody had any experience with fast respension
> of genomic DNA. Does the quality of  the phenol or the chloroform can make
> the difference?

I´ve never seen genomic DNA (neither prokaryotic nor eukaryotic) dissolving in
seconds. I agree with you that it takes hours (to overnight) at elevated temperature.

Frank



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