Green gels

[Paul N. Hengen]

> Lately my agarose gels are developing a background
> green fluorescence. They seem to turn green in front
> of my eyes under the UV transilluminator.
> Any one knows why? and how to prevent it.

Just a hunch...are you using TAE buffer?
Thoroughly clean your electrophoresis unit,
make up fresh buffer and sterilze it, or
switch to TBE buffer.

@article{Hengen1994Dectibs, 
author = "P. N. Hengen",
title = "Methods and reagents - Glowing blue gels and nuked nucleases",
journal = "Trends in Biochemical Sciences", 
volume = "19", 
number = "12", 
pages = "556-557", 
month = "December", 
year = "1994"}

Point your browser to:
ftp://ftp.ncifcrf.gov/pub/methods/TIBS/dec94.txt

The TiBS archive is on the web at
http://www-lecb.ncifcrf.gov/~pnh/readme.html

-- 

-Paul.

Paul N. Hengen, Ph.D.
mailto:pnh at sirius.com
http://www-lecb.ncifcrf.gov/~pnh/