Paul N. Hengen
pnh at SIRIUS.COM
Thu Jun 25 13:39:41 EST 1998
> Lately my agarose gels are developing a background
> green fluorescence. They seem to turn green in front
> of my eyes under the UV transilluminator.
> Any one knows why? and how to prevent it.
Just a hunch...are you using TAE buffer?
Thoroughly clean your electrophoresis unit,
make up fresh buffer and sterilze it, or
switch to TBE buffer.
author = "P. N. Hengen",
title = "Methods and reagents - Glowing blue gels and nuked nucleases",
journal = "Trends in Biochemical Sciences",
volume = "19",
number = "12",
pages = "556-557",
month = "December",
year = "1994"}
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Paul N. Hengen, Ph.D.
mailto:pnh at sirius.com
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