digesting PCR product with restriction enzymes (Question)

[Parent, Eric]

Hello all.

When digesting PCR product with restriction enzymes. Does the Taq, the oil
or whatever in the PCR inhibits the action of the restriction enzymes.  My
question is: cant you use restriction enzymes directly on the PCR products
in question or do you have to get rid of all the "junk" first.

Thanks

Eric

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Eric Parent 
Invertebrates and experimental
biology division                              
Fisheries and Oceans Canada	
Maurice-Lamontagne Institute
Mont-joli, Qc
Canada

parente at dfo-mpo.gc.ca
http://www.qc.dfo-mpo.gc.ca/iml
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