digesting PCR product with restriction enzymes (Question)

Parent, Eric ParentE at dfo-mpo.gc.ca
Fri Jun 26 11:08:29 EST 1998


Hello all.

When digesting PCR product with restriction enzymes. Does the Taq, the oil
or whatever in the PCR inhibits the action of the restriction enzymes.  My
question is: cant you use restriction enzymes directly on the PCR products
in question or do you have to get rid of all the "junk" first.

Thanks

Eric

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Eric Parent 
Invertebrates and experimental
biology division                              
Fisheries and Oceans Canada	
Maurice-Lamontagne Institute
Mont-joli, Qc
Canada

parente at dfo-mpo.gc.ca
http://www.qc.dfo-mpo.gc.ca/iml
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