PCR on yeast colony

[Frederik Boernke]

namy olivier wrote:
> 
> Hi,
> 
> I'm looking for a protocol to do PCR directly on yeast
> Saccharomyces cerevisiae colony. Does anyone know a good
> protocole?
> Thanks you so much for you help.
> Any advice would be greatly appreciated.
> Olivier
> namy at infobiogen.fr

bonjour!

I don't have a real protocol but I just pick some of the colonie with
a sterile toothpick and swirl in 5 ul ddwater. Than boil for 5 min and
spin down. Use standard PCR procedure.
This has always worked in my hands for screening two-hybrid positives.

Good luck
Ricky 

******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de