3->5 Exo

yoram gerchman yoramg2 at LEONARDO.LS.HUJI.AC.IL
Fri Jun 26 04:12:41 EST 1998


Greetings netters.
I want to shave of a 3->5 cohesive end after DraIII digestion.
Which idea seem better a T4 polymerase without nucleotides are using Pfu
polymerase? Protocols for both would be most welcome.
P.S I know fill-in would be simpler but for purpose it wont do.




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