Kozak consensus elemnt in Ecoli - toxic?

stebby at welchlink.welch.jhu.edu stebby at welchlink.welch.jhu.edu
Fri Jun 26 11:43:43 EST 1998


In article <6mtjs0$hu2 at mserv1.dl.ac.uk>,
  wgschech at med.uni-tuebingen.de wrote:
>
> Hi all,
>
> I'm planning to express a protein as a malE fusion construct in E.
> coli (XL1-blue). Currently, the cDNA is in a eukaryotic expression
> vetor and carries a Kozak consensus element. It would be convenient
> for me to this sequence element in the cDNA when transferring it to
> the other plasmid.
> Is there any evidence that a Kozak consensus element will be toxic
> or otherwise interfere with gene expression in E.coli?


I can only speak from personal experience, but I've never
noticed a problem with my constructs as I shuttle them to and
fro.  Be advised though, that the Kozak sequence in these
constructs was never "100%".  Darn close to an ideal Kozak,
but not exact.

Regards,

Steve Dahl

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