digesting PCR product with restriction enzymes (Activity Chart)

Roger Fachini rfachini at amgen.com
Fri Jun 26 18:01:46 EST 1998


In article <mwcrepeau-2606981548500001 at curt.ucdavis.edu>,
mwcrepeau at ucdavis.edu (Marc Crepeau) wrote:

>  snip
 Also, if you can get your hands
> on a copy of Biochemica No. 2 (it's a trade publication by Boehringer
> Mannheim I think) there is a good write-up on this subject on page 14. It
> includes a table that lists the activities of many common enzymes in PCR
> buffer relative to their activities in the manufacturer's buffers.
> 
> 
> Marc Crepeau
> <mwcrepeau at ucdavis.edu>
> 
> 
> In article <3B675D1AC3E1D111BC1600805FEACC6701FB57 at lauimls05.qc.dfo.ca>,
> ParentE at dfo-mpo.gc.ca ("Parent, Eric") wrote:
> 
> > Hello all.
> > 
> > When digesting PCR product with restriction enzymes. Does the Taq, the oil
> > or whatever in the PCR inhibits the action of the restriction enzymes.  My
> > question is: cant you use restriction enzymes directly on the PCR products
> > in question or do you have to get rid of all the "junk" first.

Note:
This is Biochemica No. 2, 1995.  It is available as a PDF file from
Boehringer at :
http://biochem.boehringer.com/prod_inf/biochemi/app14.pdf

-- 
Roger Fachini
Bacterial Expression
Amgen Research

The opinions expressed are solely the user's and not those of Amgen.



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