DNA recovery from TBE gel

Chen Ho An chen at bsm.bioc.ucl.ac.uk
Fri Jun 26 13:14:13 EST 1998


Alex Brands (abbrands at artsci.wustl.edu) wrote:


: My questions are;  What is the problem with isolating DNA from TBE gels?

Borate inhibits ligation if it is not removed properly.

:                    Is there some way around the problem?

Try running TAE gel at different gel concentration.

: 		   Is there some other solution to my problem?

Try TTE buffer (i.e replace borate with taurine)

1X TTE  = 89 mM TRIS, 29 mM Taurine, 0.54 mM EDTA



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