DNA recovery from TBE gel
Chen Ho An
chen at bsm.bioc.ucl.ac.uk
Fri Jun 26 13:14:13 EST 1998
Alex Brands (abbrands at artsci.wustl.edu) wrote:
: My questions are; What is the problem with isolating DNA from TBE gels?
Borate inhibits ligation if it is not removed properly.
: Is there some way around the problem?
Try running TAE gel at different gel concentration.
: Is there some other solution to my problem?
Try TTE buffer (i.e replace borate with taurine)
1X TTE = 89 mM TRIS, 29 mM Taurine, 0.54 mM EDTA
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