DNA recovery from TBE gel

[Chen Ho An]

Alex Brands (abbrands at artsci.wustl.edu) wrote:


: My questions are;  What is the problem with isolating DNA from TBE gels?

Borate inhibits ligation if it is not removed properly.

:                    Is there some way around the problem?

Try running TAE gel at different gel concentration.

: 		   Is there some other solution to my problem?

Try TTE buffer (i.e replace borate with taurine)

1X TTE  = 89 mM TRIS, 29 mM Taurine, 0.54 mM EDTA



--