Q]Broth vs. Solid Culture
vurk at hymail.hanyang.ac.kr
Mon Jun 29 20:19:35 EST 1998
I made some recombinants which have Kanamycin resistance gene.
For the screening,
I incubate the recombinant in Kanamycin(25ug/ml) Solid media.
--> After a day, I found 10 colonies are grown.
--> These 10 colonies are incubated in Kanamycin(25ug/ml) Broth.
--> After a day, I found 2 colonies are grown.
10 colonies are grown on Kanamycin Solid media(25ug/ml)
But Only 2 colonies are grown in Kanamycin Broth media(25ug/ml)
8 colonies out of 10 can Not grown in Broth, even at the same
Could you tell me the reason why ?
Laboratory of Microbiology
Department of Biology
College of Natural Science
Haengdang-dong 17 Fax : 082-02-290-0952
Seoul, South Korea E-mail: vurk at hymail.hanyang.ac.kr
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Methods