preserving cells for electroporation

Roger Fachini rfachini at amgen.com
Mon Jun 29 11:06:19 EST 1998


In article <3597AB99.4FEE98BE at public.uni-hamburg.de>, Oliver Bader
<oliver_bader at public.uni-hamburg.de> wrote:

> Hi all,
> Does anyone know a good method for preserving E.coli cells for
> electroporation once they're washed (-> salt free)?
> Does glycerol blow the fuse in the electroporator?
> oliver

Hi oliver.  We routinely resuspend in 10% glycerol.  
We store the cells at -80C or in liquid nitrogen.

-- 
Roger Fachini
Bacterial Expression
Amgen Research

The opinions expressed are solely the user's and not those of Amgen.



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