preserving cells for electroporation

[bbeitzel at biomail.ucsd.edu]

10% glycerol works fine for me.  No negative effects on the electroporator.

Brett
bbeitzel at biomail.ucsd.edu


In article <3597AB99.4FEE98BE at public.uni-hamburg.de>, Oliver Bader
<oliver_bader at public.uni-hamburg.de> wrote:

> Hi all,
> Does anyone know a good method for preserving E.coli cells for
> electroporation once they're washed (-> salt free)?
> Does glycerol blow the fuse in the electroporator?
> oliver