Affinity purification of a liver protein
mgreen at animal.ufl.edu
Tue Jun 30 10:05:49 EST 1998
We are preparing to isolate a cytosolic liver protein using affinity
chromatography. We are not sure what the degree of expression will be,
however it should be high. My question is should any preliminary
"clean-up" be performed on the cytosols prior to affinity purification.
Perhaps a size exclusion or ion-exchange column? Are there any
standardized protocols for liver tissues?
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