help

youhan at 163.NET youhan at 163.NET
Tue Jun 30 23:50:03 EST 1998


 Hi,all:
>   I have tried DD-PCR to isolate differently expressed genes between
> gastric cancer cell line and normal gastric cell line.When I sequenced
> these re-PCR fragments, it seemed that a great part of each fragment
was
> vector, only 100bp/600bp was not.Should I choose double enzymes to
digest
> all  vectors and then try RACE to amplify the full-length cDNA.If I
use
> these fragments to screen cDNA library, I am afraid many false
positive
> clones will appear which is duo to the vector part.I am really
troubled
so
> much!If i try RACE, anyone who has such experiences  please send your
> useful suggestions to youhan at 163.net
>   Thanks a lot.
>
> Best regards
> youhan

________________________________________________
»¶Ó­ÄúʹÓùãÖÝÊÓ´°Ãâ·Ñµç×ÓÓÊÏähttp://www.163.net



More information about the Methods mailing list