Fw: what to do with these dd-PCR fragments

[youhan]

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> ~{7"<~HK~}: youhan <youhan at mail.fmmu.edu.cn>
> ~{JU<~HK~}: methods at net.163.net
> ~{VwLb~}: what to do with these dd-PCR fragments
> ~{HUFZ~}: 1998~{Dj~}7~{TB~}1~{HU~} 8:07
> 
> Hi,all:
>   I have tried DD-PCR to isolate differently expressed genes between
> gastric cancer cell line and normal gastric cell line.When I sequenced
> these re-PCR fragments, it seemed that a great part of each fragment was
> vector, only 100bp/600bp was not.Should I choose double enzymes to digest
> all  vectors and then try RACE to amplify the full-length cDNA.If I use
> these fragments to screen cDNA library, I am afraid many false positive
> clones will appear which is duo to the vector part.I am really troubled
so
> much!If i try RACE, anyone who has such experiences  please send your
> useful suggestions to youhan at 163.net
>   Thanks a lot.
> 
> Best regards
> youhan