Urgent help for plasmid purification

Todd Serulneck serulneck at CBR.MED.HARVARD.EDU
Sun Mar 1 23:13:42 EST 1998


Hi! Guys:

Help need for plasmid DNA purification.

I am purifying plasmid DNA using Promega miniprepa DNA Purification
System
from Novablue cell transformated by Clontech pEGFP-N1 containing the
insert. Some strange things happened, I really got the plasmid with
insert, but allways companied by
a 580 bp DNA band. Since the plasmid DNA is to be used for transfection,
so I got to figure out  what  it is.
Does anybody have experience about this?

Please help!
 
 
 




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