Purification of end labelled oligo probes?
Ransom Hill Bioscience
visla at ix.netcom.com
Mon Mar 2 19:43:53 EST 1998
In article <6de4dn$2co at is.bbsrc.ac.uk>,
Fred Bloggs <Fred.Bloggs at hri.ac.uk> wrote:
>Is it worth trying to purify small (20bp) end labelled probes? I have had a problem with high
background so I would like to purify the probes. I have tried ethanol precipitation but that
was not very good.
Freie, B. and S. Larsen. 1991. Oligonucleotide Purification in Milligram Quantities.
BioTechniques, Vol 10. No. 4, pp. 420-422.
Just scale it down, i.e. use a smaller cartridge. We have employed this method for years and it
works beautifully. We use Sep-paks.
Mike MacDonell, Ph.D.
Chief Scientific Officer
Ransom Hill Bioscience, Inc.
More information about the Methods