Purification of end labelled oligo probes?

rebecca rebsilver at usa.net
Tue Mar 3 07:44:01 EST 1998



ISOAI Atsushi wrote:

> In article <6de4dn$2co at is.bbsrc.ac.uk>, Fred.Bloggs at hri.ac.uk says...
> >
> >Is it worth trying to purify small (20bp) end labelled probes?
> > I have had a problem with high background so I would like
> >to purify the prob
>
> Please try QIAquick Nucleotide Removal Kit. Its information
> is avaiable at;
> http://www.qiagen.com/catalog/Chapter8a.html
>

Hi,I have done this as well (15-20 nt for EMSAs) and can vouch for it.
It is fast, clean, and easy.
Reb

> --
> ISOAI Atsushi, Ph.D. <aisoai at agc.co.jp>
> Research Center, Asahi Glass Co.LTD.
> http://www.yk.rim.or.jp/%7Eaisoai/molbio-j.html








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