Urgent help for plasmid purification

Todd Serulneck serulneck at CBR.MED.HARVARD.EDU
Wed Mar 4 15:18:05 EST 1998


Thank you for reply!

I re-tranformated novablue cell with pEGFP-N1 without extra band (580
bp)
or plasmid with insert and strange band. After miniprep,  that strange
band
shaw up in all of samples.

Maybe the novablue cell has been contaminated.

I should try  to re-transformate another strain.
 
 

Thank you, Guys!
 
 




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