Brent/Clontech yeast 2-hybrid screen: recombination/rearrangements.

Vladimir Svetlov svetlov at oncology.wisc.edu
Wed Mar 4 12:30:30 EST 1998


In article <6d633q$81j$1 at nntp3.u.washington.edu>, polliwog at u.washington.edu
(S. Findley) wrote:


> Also: is there a superior strain to KC8 (E.coli) for rescuing 
> Trp plasmids via auxotrophy?

I used to outgrow positive yeast colonies in Trp- medium for three cycles
[50 ul of OD600=1 culture into 15 ml of medium -> to OD600=1] and after
that 80%+ of the colonies of E.coli were containing TRP1 plasmid. Rescueing
in trp- E. coli strains never been any better than that.

Regards,
V.

-- 
Vladimir Svetlov
McArdle Lab for Cancer Research
Dept. Oncology
UW-Madison
1400 University Ave.
Madison, WI 53706



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