dTTP -> dUTP problems in PCR

Nico van Belzen belzen at hema.fgg.eur.nl
Wed Mar 4 05:24:11 EST 1998


Hi all, 

(posting this for a colleague) 
In order to prevent contamination, I want to use dUTP/UDG (uracyl 
glycosylase) in all diagnostic PCR systems. However, sometimes the 
reaction is not working when I change from dTTP to dUTP. I tried to 
increase the dUTP concentration, this did not help. Trying other 
brands of Taq polymerase does not work. Does somebody know a solution 
for this problem? 

Best regards, 

Nico van Belzen (belzen at hema.fgg.eur.nl) 




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