Cloning cDNA directly from PCR products

Christophe Jacob jacob at scbiol.u-nancy.fr
Thu Mar 5 14:16:45 EST 1998


Hello,

I'd like to clone several PCR fragments into plasmid vector. Their size is
ranging from 200 to 700 pb.
Is it possible to use them for ligation after a step of purification (
PCIH, CI) and precipitation then digestion with the restriction enzyme,
without electrophorezing them on agarose gel.

Best regards,

Christophe



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