Cloning cDNA directly from PCR products

Christophe Jacob jacob at
Thu Mar 5 14:16:45 EST 1998


I'd like to clone several PCR fragments into plasmid vector. Their size is
ranging from 200 to 700 pb.
Is it possible to use them for ligation after a step of purification (
PCIH, CI) and precipitation then digestion with the restriction enzyme,
without electrophorezing them on agarose gel.

Best regards,


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