Phosphorylation of library proteins

Sylke Buder buder at ITA.FHG.DE
Thu Mar 5 05:13:33 EST 1998


Does anybody have experience in phosphorylation of library proteins? We
want to modify the yeast two-hybrid system for the identification of
tyrosine-phosphorylated proteins that would bind to SH2-Domains on our
bait protein.
Does anybody know a useful kinase which promotes the tyrosine
phosphorylation of library proteins?
Thanks in advance.



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