Cloning cDNA directly from PCR products
knben at ttacs.ttu.edu
Thu Mar 5 15:22:04 EST 1998
Christophe Jacob <jacob at scbiol.u-nancy.fr> wrote in article
<jacob-0503982016450001 at morchella.scbiol.u-nancy.fr>...
> I'd like to clone several PCR fragments into plasmid vector. Their size
> ranging from 200 to 700 pb.
> Is it possible to use them for ligation after a step of purification (
> PCIH, CI) and precipitation then digestion with the restriction enzyme,
> without electrophorezing them on agarose gel.
> Best regards,
What you are talking about is a mini-library construction. Either Phenol
chloroform it or use one of these PCR clean-up kits and use for
sub-cloning. Good luck!
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