Yeast 2 hyb screening

S. Findley polliwog at u.washington.edu
Thu Mar 5 15:39:51 EST 1998


Yes, direct plating onto selective medium when using the Brent/Clontech
system is definitely not a good idea. (LexA-bait, gal-dependent cDNA
expression vector etc.)

I tried this once:
I think I plated what would have been I think 2 million primary
transformant onto H-U-T-L- Gal/Raf plates and got a single colony which
survived the selection. Not good.

-SDF
-- 
Seth D. Findley               polliwog at u.washington.edu
Department of Biochemistry    Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195



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