Ideal system for (plant) protein expression in yeast

[bipin k. dalmia]

On 5 Mar 1998 17:40:39 GMT, "Benan Dincturk" <knben at ttacs.ttu.edu>
wrote:

>Hello netters
>
>E. coli expression does not seem to work for my huge protein. So while I am
>playing with E. coli vectors, I plan to start with yeast expression. Who
>has experience with yeast? Can you suggest some vectors with strong
>promoters? What are the important points in yeast transformation? I know
>that is the thoughest part usually. I am a beginner so every suggestion
>will be appreciated. Thank you all.
>
>Benan

try Invitrogen's Pichia pastoris expression system. works extremely
well in our hands. 

bip


Bipin K. Dalmia, Ph.D.
Protein Core Facility
Pioneer Hi-Bred International, Inc.
Johnston, Iowa 50131

dalmiabk at phibred.com