In vitro translation systems

Benan Dincturk knben at ttacs.ttu.edu
Sun Mar 8 15:52:07 EST 1998


I am thinking of using an in vitro translation system to express my spinach
protein. The protein is 160 Kda. I am thinking of yeast system but to get
rid of my an early stop codon that might have been created by a PCR cloning
of the gene, I think this is a good idea. Has anybody used such systmes
before? For a plant protein will wheat germ system be better? Can you tell
me about your experience and are they easy to use?

knben at ttacs.ttu.edu



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