cells BRL(DE3)pLysS

ruben.varela-calvino at kcl.ac.uk ruben.varela-calvino at kcl.ac.uk
Mon Mar 9 07:38:54 EST 1998


Hi everybody:

I'm using the pET vector for cloning and expressing my proteins. The problems
come when I try to transform the BRL(DE3)pLysS cells. I follow the Novagen
protocol (and cells) but I only get 4-5 colonies and not in the middle of the
plate (I use LB plates with Amp, and then spread Chloramphenicol and
Tetracycline on then, leave dry before plate the transformation).
Any suggestion to improve the trasnformation?
Thanks in advance.
Ruben Varela-Calvino

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