sequencing PCR products
tyr-2 at bones.biochem.ualberta.ca
Tue Mar 10 11:31:59 EST 1998
In article <Pine.GSO.3.94.980310095334.12303A-100000 at tin>, "Miss. V.M.
Hodges" <vhodges at hgmp.mrc.ac.uk> wrote:
> has anyone got a good method for manual sequencing of PCR products
Posted and mailed.
We have been doing sequencing of PCR products for a number of years using
the dsDNA cycle sequencing kit from Gibco LifeSciences (no affil). Have
gotten good sequence from a 293 bp PCR fragment without the need to
pre-purify the PCR-generated DNA. Additional benefit is you only need 30
ng/kb of DNA in order to sequence (in your case, 15-20 ng of your 500bp
product should work fine).
Sorry the suggestion had to be another kit.
Karl the hepB guy
More information about the Methods