Native polyacrylamide gel question

[Bruce G. Allen]

On 10 Mar 1998 ccc6 at Lehigh.EDU wrote:

Christine,
What is the isoelectric point of the protein? It may have the wrong net
charge, or insufficient net charge, at the pH of the gel buffers to move
when the current is applied. You could try gels at various pHs (alkaline,
neutral, acid.

Cheers, 
Bruce

Bruce G. Allen, Ph.D.                          Phone: 514-376-3330 (3591) 
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