Native polyacrylamide gel question

Bruce G. Allen allen at cardio.ICM.UMontreal.CA
Wed Mar 11 21:05:25 EST 1998


On 10 Mar 1998 ccc6 at Lehigh.EDU wrote:

Christine,
What is the isoelectric point of the protein? It may have the wrong net
charge, or insufficient net charge, at the pH of the gel buffers to move
when the current is applied. You could try gels at various pHs (alkaline,
neutral, acid.

Cheers, 
Bruce

Bruce G. Allen, Ph.D.                          Phone: 514-376-3330 (3591) 
Institut de Cardiologie de Montreal            FAX:   514-376-1355
Centre de Recherche                            EMAIL: allen at icm.umontreal.ca 
5000, rue Belanger est
Montreal, Quebec
Canada
H1T 1C8




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