msp I digestion

X. Y. Zheng xi-zheng at tamu.edu
Thu Mar 12 11:48:09 EST 1998


Dr GJS Jenkins wrote:
> 
> Does anyone know why msp I would fail to cut DNA
> We have had trouble cutting genomic DNA and PCR
> product with this enzyme. I am aware of the methylation
> problem, but this does not explain the PCR cutting problems.

Hi,

I had the same problem with MspI from different sources.  Only one time
I had complete digestion of my PCR product purified by Spin modul from
Bio 101, Inc. But I couldn't repeat it.  What I found was always
imcomplete digestion.  

Cheers

XY Zheng



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