phagosome purification

Lestrate Pascal Pascal.Lestrate at
Fri Mar 13 08:44:01 EST 1998

I would like isolate a pathogen-phagosome from infected Hela cells but we
have only in BL3 the possibility to realize centrigugation at 10000 rpm. Is
it possible to me to isolate a such compartment? Have you a protocol for
Thanks you


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