Help. Factor Xa digestion

Jongrak Kittiworakarn g3937506 at STUDENT1.MAHIDOL.AC.TH
Fri Mar 13 22:19:58 EST 1998


 Dear all:

I have expressed my peptide of interest with GST fusion, but it was in
the inclusion.
I have some problem with the refolding of this product. So, I think
about cleaving my peptide out when it was in the present of guanidine
HCl.

Anyone know that if Factor Xa can make a specific digestion in the
guanidine HCl. The condition I may use is 0.5M guanidine HCl in 50mM
Tris-HCl pH 8.0.

TIA

Jongrak

--
-----------------------------------Mr. Jongrak Kittiworakarn
                                   g3937506 at student.mahidol.ac.th

     Inst. of Science and Technology for Research and Development
     Mahidol University (Salaya Campus)
     Salaya, Nokornprathom, THAILAND.
     Tel. 001-66-2-441-9003-7 ext.1277,1278,1279
     Fax. 001-66-2-441-9906, 001-66-2-441-1013
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