Yeast and GFP
John R. Thompson
jrt at home.com
Sat Mar 14 10:46:40 EST 1998
You don't give many details. What instrument and filter sets are you
using? What media and growth conditions? Which promoter are you
using? Describe your GFP variant. Is it codon-optimized for yeast or
Using fluoroscein filters I've seen a significant amount of
fluorescence in the spent medium (synthetic complete) from control
(non-GFP expressing) cells. Thus, washed cells show the
GFP-associated fluorescence better.
Merck & Co.
Paula <ssteinh at gwdg.de> wrote:
>Does anybody know, why in my yeast there is no fluorescence in my
>GFP-transformed yeast. I have used the strain UCC3505 and transformed
>with pYX242 EcoRI, XbaI inserted pEGFP-N1.
>Any suggestions ?
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