degenerate oligo PCR
han at biogate.com
Sun Mar 15 03:13:14 EST 1998
You can check out the website of bionet.(http://www.bionet.com ?). Then you
can search the achieve database and find what you want.
gut hormone lab
J. Casey Lippmeier wrote in message ...
> A few weeks ago someone on this group posted a well written
>outline of some of his/her observations on how to conduct successful PCRs
>using degenerate oligos. I printed it and promptly lost it. One specific
>observation that I can remember from the post concerned the use of
>degenerate oligos at a mass larger than what's normally used by a factor
>of it's degeneracy (ie. if using a degenerate oligo with a degeneracy of
>64 and one normally uses 10 nmol of primer one should now use 640 nmol of
>degenerate primer). There were a number of other useful suggestions in
>this posting that I would like to try (the aforementioned one worked
>particularly well), but I can't remember them. If anyone saved this post
>please forward it to this email address or "gene1 at clark.net".
> Is this newsgroup archived anywhere? Any information on this
>would be useful as well (excuse my ignorance on the archiving issue, I
>haven't had this kind of need yet).
>-J. Casey Lippmeier
More information about the Methods