degenerate oligo PCR

Han han at
Sun Mar 15 03:13:14 EST 1998


You can check out the website of bionet.( ?). Then you
can search the achieve database and find what you want.

Good luck.

Huang Zhong
gut hormone lab

J. Casey Lippmeier wrote in message ...
>      Hello,
>          A few weeks ago someone on this group posted a well written
>outline of some of his/her observations on how to conduct successful PCRs
>using degenerate oligos.  I printed it and promptly lost it.  One specific
>observation that I can remember from the post concerned the use of
>degenerate oligos at a mass larger than what's normally used by a factor
>of it's degeneracy (ie. if using a degenerate oligo with a degeneracy of
>64 and one normally uses 10 nmol of primer one should now use 640 nmol of
>degenerate primer).  There were a number of other useful suggestions in
>this posting that I would like to try (the aforementioned one worked
>particularly well), but I can't remember them.  If anyone saved this post
>please forward it to this email address or "gene1 at".
> Is this newsgroup archived anywhere?  Any information on this
>would be useful as well (excuse my ignorance on the archiving issue, I
>haven't had this kind of need yet).
>-J. Casey Lippmeier

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