degenerate oligo PCR

Han han at biogate.com
Sun Mar 15 03:13:14 EST 1998


Hi,

You can check out the website of bionet.(http://www.bionet.com ?). Then you
can search the achieve database and find what you want.

Good luck.

Huang Zhong
gut hormone lab
k.u.leuven
belgium

J. Casey Lippmeier wrote in message ...
>
>      Hello,
>
>          A few weeks ago someone on this group posted a well written
>outline of some of his/her observations on how to conduct successful PCRs
>using degenerate oligos.  I printed it and promptly lost it.  One specific
>observation that I can remember from the post concerned the use of
>degenerate oligos at a mass larger than what's normally used by a factor
>of it's degeneracy (ie. if using a degenerate oligo with a degeneracy of
>64 and one normally uses 10 nmol of primer one should now use 640 nmol of
>degenerate primer).  There were a number of other useful suggestions in
>this posting that I would like to try (the aforementioned one worked
>particularly well), but I can't remember them.  If anyone saved this post
>please forward it to this email address or "gene1 at clark.net".
> Is this newsgroup archived anywhere?  Any information on this
>would be useful as well (excuse my ignorance on the archiving issue, I
>haven't had this kind of need yet).
>
>Hopefully,
>
>-J. Casey Lippmeier
>
>





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