cresol ution

["David Brayford ", B&B-BMB]

Hi All
Can anyone please advise on appropriate concentrations of cresol red and 
sucrose to include in PCR reactions, in order to facilitate gel loading? 
 Following published protocols I tried 0.2mM cresol red and 12% sucrose 
(final conc.) but got no amplification and no dye visible on the gel. 
 Controls were lovely.  I guess the sucrose is the problem.  Any advice 
appreciated.
Ta.
Dave
d.brayford at cabi.org