"David Brayford ", B&B-BMB
D.BRAYFORD at cabi.org
Tue Mar 17 05:05:36 EST 1998
Can anyone please advise on appropriate concentrations of cresol red and
sucrose to include in PCR reactions, in order to facilitate gel loading?
Following published protocols I tried 0.2mM cresol red and 12% sucrose
(final conc.) but got no amplification and no dye visible on the gel.
Controls were lovely. I guess the sucrose is the problem. Any advice
d.brayford at cabi.org
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